Survivin, phosphorylated (Ser20) (Apoptosis Inhibitor 4, Apoptosis Inhibitor-4, API4, Apoptosis Inhibitor Survivin, Baculoviral IAP Repeat-containing 5, BIRC5, BIRC-5, EPR1, EPR-1, IAP4, SVV, TIAP)

Size

£594.00

SKU:: S8500-01P
Application:: WB
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide surrounding the phosphorylated serine 20 of the human Survivin protein. [Swiss-Prot #O15392]
Format:: Supplied as a liquid in PBS, 30% Glycerol, 0.09% sodium azide.
Extra Details:: Survivin is a structurally unique inhibitor of apoptosis (IAP) that has been linked to protection from apoptosis, control of cell division, and cellular adaptation to stress. In order for survivin to provide this cytoprotection an interaction with at least one other IAP, such as XIAP, is necessary. It has recently been shown that Survivin is phosphorylated at Ser20 by protein kinase A (PKA) in the cytosol but not in the mitochondria. This phosphorylation on Ser20 disrupts the binding of cytosolic survivin to XIAP and therefore diminishes survivin's cytoprotective properties. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.