S8402-07
Surfactant Protein D (SP-D)
Size
£819.00
- SKU:
- S8402-07
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified rat surfactant protein D.
- Clone:
- 6A621 (VIF9)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- SP-D belongs to the collectin familiy. These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions. They are structurally similar to the ficolins although they make use of different CRD structures: C-type lectin domain for the collectins. The anti-microbial effector mechanisms of SP-D are direct opsonization, neutralization, and agglutination. Thus limiting the infection and concurrently orchestrating the subsequent adaptive immune response. The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type II cells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointeststinal tract and in epithelial cells of exocrine glands. SP-D synthesis and secretion increase significantly after inflammatory stress. Increased amounts of SP-D in lavage and tissue, particularly in type II pneumocytes, in Clara cells and in hyperplastic goblet cells are found in inflamed lungs. The localization of SP-D in endocytic vesicles and in lysosomal granules of alveolar macrophages suggests that a receptor-mediated uptake occurs. SP-D binds to apoptotic neutrophils and enhances their clearance by alveolar macrophages. Does not block or interfere with the calcium-dependent carbohydrate binding activity of the lectin domain and utilizes its C-type lectin domain to attach to surface glycans on pathogens (like bacteria, fungi, and viruses). S8402-07 in assays allows you to detect or capture SP-D without disrupting its natural ability to bind to these target organisms Applications: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested Recommended Dilutions: Western Blot: 1:10 using a non-reduced sample and SDS-PAGE. ELISA: Capture or detection antibody. Immunohistochemistry (Paraffin): 1:10 on rat lung only. Cannot be used on paraffin sections of human lung. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice