S8060-25-ML550
Superoxide Dismutase, Mn (SOD Mn) (MaxLight 550)
Size
£1,023.00
- SKU:
- S8060-25-ML550
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Manganese superoxide dismutase (MnSOD).
- Clone:
- 2Q339 (MnS-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Superoxide dismutases (SOD) are found in all aerobic organisms. Their physiological function appears to be to protect cells from free radicals by scavenging superoxide anions. They catalyse the dismutation of superoxide anions to oxygen and hydrogen peroxide. SOD(Mn) is located primarily in mitrochondria and is thought to be of prokaryote origin. SOD(Mn) is a tetramer of identical subunits of MW 25kD by SDS-PAGE. Applications: Suitable for use in Immunohistochemistry , Immunocytochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10ug/ml using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 (higher triton is only used on thick sections; blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4°C. Wash 3x10’ in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites. Immunocytochemistry (cell smears): 0.5-10ug/ml. Western Blot: Under reducing conditions a band at ~25kD will be identified. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice