S7975-88
Stromal Interaction Molecule 1 (STIM1)
Size
£655.00
- SKU:
- S7975-88
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 24aa near the carboxy terminus of human STIM1.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++- release-activated Ca++ (CRAC) channels (reviewed in 1). These channels are made up of the pore-forming subunit ORAI1 (2) and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store (3,4). A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry (5). At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2 ug/ml. Immunohistochemistry: 2.5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse thymus tissue lysate Human spleen tissue lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice