S7975-13C

Stromal Cell Derived Factor 1alpha (SDF1 alpha, SDF1a, SDF-1a, Chemokine (C-X-C Motif) Ligand 12, CXCL12, hIRH, Pre-B cell Growth-stimulating Factor, PBSF, SCYB12, TLSF-a, TPAR1) (Biotin)

Size

£657.00

SKU:: S7975-13C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant mouse SDF-1 alpha.
Format:: Supplied as a lyophilized powder in PBS, pH7.2. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Extra Details:: SDF-1 alpha, otherwise known as CXCL12a, a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons. SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.25-1ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.