S7973-73B
Streptavidin, His-Tag, aa25-183, Recombinant, Human
Size
£512.00
- SKU:
- S7973-73B
- Molecular Weight:
- 17.5
- Purity:
- ≥95% by SDS-PAGE under reducing conditions
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- CAS Number:
- 9013-20-1
- Format:
- Supplied as a liquid in 20mM Tris-HCl, pH 7.5.
- Extra Details:
- Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa25-183 of Streptomyces avidinii, with a His Tag, expressed in E. coli. Uniprot/Accession: CAA27265.1 Amino Acid Sequence: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice