S7973-30
Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)
Size
£338.00
- SKU:
- S7973-30
- Molecular Weight:
- 25
- Purity:
- ≥98% by SDS-PAGE and HPLC.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS, 1mM DTT. Reconstitute with sterile ddH2O to 0.1-1.0mg/ml. DO NOT VORTEX. Add buffer containing carrier protein for long term storage.
- Extra Details:
- Stem Cell Growth Factor alpha and beta are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGFb is a 25kD polypeptide containing 227 amino acid residues. Endotoxin: ≤0.1ng/ug (1EU/ug). Biological Activity: Determined by the dose dependant stimulation of the proliferation of human TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed is ≤7ng/ml. Amino Acid Sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice