STAT 6 (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6)

Size

£581.00

SKU:: S7972-42A
Purity:: Purified by Protein A and peptide affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 620 of human Stat6
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol
Extra Details:: When activated by Janus kinases Stat6 is transported to the nucleus where it regulates cytokineinduced gene expression. Required for responsiveness to IL-4 and IL-13 (1,2), Stat6 is activated via phosphorylation at tyrosine 641 (3,4). In addition to its role in mediating IL-4 responses, Stat6 is activated in B cell lines by IFN-alpha, where it forms transcriptionally active complexes with Stat2 and p48 protein (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.