S7972-10G

STAT 5a (Signal Transducer and Activator of Transcription 5a)

Size

£800.00

SKU:: S7972-10G
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa774-794, SLDSRLSPPAGLFTSARGSLS, of human STAT5a.
Clone:: 7H57
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Signal Transducer and Activator of Transcription 5a (STAT5a) is a member of the STAT family of proteins that mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT5a and STAT5b are encoded by separate genes and share 93% amino acid identity. Applications: Suitable for use in Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml using immersion fixed K562 human chronic myelogenous leukemia cell line Flow Cytometry: 0.25ug labels 10e6 cells using jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.