S7972-07Q

STAT5A/B, phosphorylated (Tyr694/699)

Size

£800.00

SKU:: S7972-07Q
Application:: WB
Purity:: Tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B. Cellular Localization: Cytoplasm. Nucleus. Epitope: pTyr694/699. Species sequence homology: chimpanzee, bovine and rat.
Clone:: A11W
Format:: Supplied as a liquid 0.05% sodium azide.
Extra Details:: The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation. Applications: Suitable for use in Western Blot. Not suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF. Optimal dilutions to be determined by the researcher. Positive Control: PDGF treated 3T3/NIH cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice