S7972-07J

STAT 5, phosphorylated (Signal Transducer and Activator of Transcription 5)

Size

£709.00

SKU:: S7972-07J
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: A synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of mouse STAT5. Species sequence homology: human, bovine, sheep, and rat STAT5 proteins.
Clone:: 6A188
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Extra Details:: STATs (signal transducers and activators of transcription) were originally identified as DNA-binding proteins essential to interferon IFNa- and IFNg-regulated gene expression. Since then, several additional mammalian STATs, a Drosophila STAT (D-STAT or Marelle), and a STAT-like protein from Dictyostelium discoideum have been recognized. In unstimulated cells STAT proteins exist largely as latent cytoplasmic transcription factors. Treatment of cells with cytokines or certain growth factors results in the tyrosine phosphorylation (via Janus kinases for cytokines) of specific STATs causing them to form homodimers or heterodimers that enter the nucleus. In the nucleus, STAT dimers bind DNA and activate transcription of distinct target genes. Although several different STATs may be activated in response to a given ligand, there is often a striking specificity in the pattern of STAT activation. Mammalian STAT proteins share several conserved structural and functional domains as illustrated below: STAT5 (mammary growth factor) was originally isolated as a tyrosine phosphorylated DNA binding protein from prolactin stimulated mammary tissue and IL-3 stimulated myeloid cells. Subsequently, two highly homologous (~96%) genes, Stat5a and Stat5b, were identified in mouse.7-8 Both STATs are expressed in most tissues of virgin and lactating mice although differential accumulation of STAT5a and STAT5b mRNAs occurs in muscle and mammary tissues. STAT5 activation has been observed in response to IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and prolactin (PRL). STAT5a and STAT5b proteins recognize the GAS (gamma interferon activating sequence) site TTCNNNGAA with the preferred DNA-binding consensus site deduced as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein, isolated from a variety of cultured cells, indicates that the protein migrates with a molecular weight of ~95kD. C-terminal truncated forms of the STAT5a protein have also been reported. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml ELISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Controls: STAT5 transfected 293 cells expressing either wild type or inactive JAK1 kinase, A431and K562 cell lysates prepared before and after treatment with epidermal growth factor. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice