S7970-86
STAT 2 (Signal Transducer and Activator of Transcription 2)
Size
£800.00
- SKU:
- S7970-86
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Fusion Protein corresponding to residues 672-925 of murine STAT2.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
- Extra Details:
- Applications: Suitable for use in Western Blot, Immunoprecipitation, EMSA. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ul. 3T3 cell lysate probed with anti-STAT2 (0.5ug/ul). Immunocytochemistry: Positive immunostaining reported on fresh cells fixed with 3.7% formaldehyde and permeabilized with 0.2% Triton1. EMSA: Supershift STAT2 containing complexes Optimal dilutions to be determined by the researcher. Positive Antigen Control: 3T3 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice