S7950-56F

SI-CLP (Chitinase Domain-containing Protein 1, Stabilin-1-interacting Chitinase-like Protein, CHID1, GL008, PSEC0104, SB139)

Size

£745.00

SKU:: S7950-56F
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Fusion protein corresponding to aa24-73 fragment conjugated to GST.
Clone:: 1C11
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: SI-CLP is a 39kD member of the family of Glyco_18-domain-containing proteins. These chitinase-like proteins are involved primarily in Th2-associated disorders and tumor progression. Chitinase-like proteins are soluble mediators of cell differentiation, proliferation, activation, migration, and adherence. Chitinase like protein SI-CLP is a stabilin-1 interacting partner. SI-CLP gene expression was up- regulated by IL-4 and dexamethasone on the mRNA and protein levels in primary human macrophages in parallel with the expression of stabilin-1. The major site of SI-CLP intracellular localization is in Lamp1-positive and secretion commited CD63+ secretory lysosomes. The endocytic/sorting receptor stabilin-1 is a highly specific intracellular sorting receptor for SI-CLP. SI-CLP directly binds to, and colocalizes with stabilin-1. The lysosomal localization of SI-CLP in stabilin-1-negative macrophages, however, indicates that stabilin-1 may not be the only receptor mediating trafficking of newly synthesized SI-CLP. In vivo, this monoclonal antibody recognizes SI-CLP in the cellular fraction of bronchoalveolar lavage specimens obtained from patients with chronic inflammatory disorders of the respiratory tract and in peripheral-blood leukocytes (PBLs) from these patients as well as healthy donors. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:50 Immunofluorescence: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice