S7950-55B
Stabilin 1 (FEEL-1, CLEVER-1) (Biotin)
Size
£863.00
- SKU:
- S7950-55B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant protein corresponding to Gly2208-Val2305 from human Stabilin-1.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor 1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin, multiple EGF-like and laminin type EGF-like, and a link domain. The short cytoplasmic tail interacts with CGAs which are cargo adaptors that link cargo into the clathrin-coated pits. Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands including acetylated LDL, SPARC, AGE, and SI-CLP. By alternative splicing, a soluble Stabilin-1 containing a 58 residue substitution between amino acid residues 746 and 803, followed by truncation, has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Stabilin-1. The detection limit for rhStabilin-1 is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1% cross-reactivity with rhStabilin-2. Optimal dilutions should be determined by each laboratory for each application. Reconstitution: Reconstitute with sterile Tris-buffered saline pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% BSA. If 1 mL of buffer is used, the antibody concentration will be 50ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice