S7900-16

SCD4 (Stearoyl-CoA Desaturase 5, Acyl-CoA-desaturase 4, HSCD5, Stearoyl-CoA 9-desaturase, SCD5, ACOD4)

Size

£754.00

SKU:: S7900-16
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 18aa of mouse SCD4, N-terminal.
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Stearoyl-CoA desaturase (SCD, also known as Delta-9 desaturase, EC 1.14.99.5) is an iron containing membrane enzyme. It catalyzes the rate limiting step in the biosynthesis of mono-unsaturated fatty acids. The enzyme inserts a cis double bond between the C9 and the C10 positions in the acyl-CoA derivatives of saturated fatty acids such as palmitic acid and stearic acids to produce palmitoleoyl- and oleoyl-CoA, respectively. Mono-unsaturated fatty acids play an important role in many processes, including energy metabolism (triacylglycerol storage in adipose tissues), anti- oxidation, lipid activated signal transduction, apoptosis, mitogenesis in certain tumors, and activate certain hormone receptors. SCD genes are tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. A number of SCD genes have been identified (two SCD genes in rat, and SCD1 and SCD5 in human, and SCD1-4 in mouse). All mouse SCD genes are located on chromosome 19. The SCD-gene products are integral membrane proteins with four TM domains containing a novel sterol regulatory element binding protein (SREBP) binding site. The SCD enzymes have a 30aa N-terminal sequence responsible for their rapid degradation and short half-life of 3-4 hours. Most prominent expression of SCD1 is in lipogenic tissues (liver and adipose), SCD2 in brain, SCD3 in skin, preputial and harderian gland, and SCD4 in the heart. SCD-4: The enzyme SCD4 (mouse 352aa, ~37-40kD; 79% identity with SCD1-3) expressed is restricted in the heat. Liver X receptor alpha (LXR-alpha) agonists and high carbohydrate fat-free diet induces SCD4 expression. Unlike SCD1, SCd4 expression is no repressed by dietary polyunsaturated fatty acids. Leptin deficient ob/ob mice show elevated levels of SCD4 in the heart, and leptin treatment reduced SCD4 levels without affecting SCD1 and SCD2. SCD1 deficient mice showed elevated levels of SCD4. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice