Six-transmembrane epithelial antigen of the prostate-1 (STEAP1, six transmembrane epithelial antigen of the prostate 1, Metalloreductase STEAP1, MGC19484, PRSS24, Six-transmembrane epithelial antigen of prostate 1, STEAP)

Size

£856.00

SKU:: S7500-05
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Met1-His72 from human Six-transmembrane epithelial antigen of the prostate-1, expressed in E. coli
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40kD protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell-cell junctions between prostate secretory epithelium, and upregulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339aa in length. It contains a ferric oxidoreductase domain (aa119-265) that includes transmembrane sequences (# 2, 3, 4 and part of 5, out of 6 sequences). Two splice forms diverge at aa255, terminating after aa58 or 259. Over aa1- 71, human and mouse STEAP1 share 68% aa identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using LNCaP human prostate cancer cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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