S7500-02
STEAP4 (Six Transmembrane Epithelial Antigen of Prostate 4, STEAP Family Member 4, Metalloreductase STEAP4, SixTransMembrane Protein of Prostate 2, STAMP2, TIARP, Tumor Necrosis Factor alpha-induced Protein 9, TNFAIP9)
Size
£800.00
- SKU:
- S7500-02
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-His459 from human STEAP4 expressed in NS0 cells.
- Clone:
- 8H178
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human STAMP2, also called Steap4 or TIARP (TNF-alpha-induced adipose-related protein), is a 55kD, 459aa, 6-transmembrane (TM) member of the Steap family of metalloreductases. It is induced by androgens in androgen-regulated prostate cells, and is upregulated during adipocyte differentiation by TNF-alpha. Full-length human and mouse STAMP2 share 80% aa identity. A 283aa variant lacks a portion of the oxidoreductase domain and three TM domains. STAMP2 is present in the Golgi complex and early endosomes in addition to the cell surface. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells using Human STAMP2 transfected HEK293 human cell line. Optimal dilutions to be determined by the researcher. Positive Control: A549 cells Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice