S6610-11
Stage Specific Embryonic Antigen 4 (SSEA4)
Size
£889.00
- SKU:
- S6610-11
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 2102Ep human embryonal carcinoma cell line
- Clone:
- 8H218
- Format:
- Supplied as a lyophilized powder from 50mM MES, pH 6.5, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells. Using NTera-2 human testicular embryonic carcinoma cell line Immunocytochemistry: 8-25ug/ml using immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice