S6600-90
SspI
Size
£307.00
- SKU:
- S6600-90
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Extra Details:
- 5'-A A T^A T T-3' 3'-T T A^T A A-5' Concentration: 10u/ul Source: Sphaerotilus species Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubate at 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 15-fold overdigestion (15u/ug lambda DNA x 1 hour) with SspI (see Star Activity). Ligation/Recutting Assay: After 10-fold overdigestion (2u/ug DNA x 5 hours) with SspI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.3uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Star Activity: An excess of enzyme (20u/ug DNA x 1 hour) may result in star activity. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Number of Recognition Sites in DNA: Lambda: 20 PhiX174: 1 M13mp18/19: 6 pBR322: 1 pUC18/19: 1 pUC57: 1 pTZ19R/U: 2 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 2 pACYC184: 2
- Shipping Conditions:
- Blue Ice