S6570

SR Proteins (Non-snRNP Splicing Factor)

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£761.00

SKU:: S6570
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The epitope has been mapped to a 40 amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate.
Clone:: 3G269
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Extra Details:: SR proteins are a highly conserved family of arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro-a particularly interesting example is the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins. SR proteins appear to be recruited from nuclear “speckles”, in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus. The epitope detected by the antibody has been termed the “alternating arginine domain” and is composed almost exclusively of argnine alternating with glutamate and aspartate. Antibody targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription. Epitope is conserved in metazoans. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 0.5-2ug/ml ELISA: 0.1-1ug/ml Western Blot: 3-5ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: HeLa cell, nuclear extracts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice