SREBF2 (Sterol Regulatory Element-binding Protein 2, SREBP-2, Class D Basic Helix-loop-helix Protein 2, bHLHd2, Sterol Regulatory Element-binding Transcription Factor 2, BHLHD2, SREBP2)

Size

£704.00

SKU:: S6505-18
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the internal region of human SREBF2.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Extra Details:: SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.2 SREBP-2 is ubiquitously detected in various tissues. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68kD) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:150 Immunohistochemistry (FFPE): 1:40. Requires HIER in pH 6.0 citrate buffer. Western Blot: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice