SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2)

Size

£642.00

SKU:: S6505-08R
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide C-KMFEDYPKSRK, from the C-Terminus of human SRD5A2.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA:1:16000 Western Blot:1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Testis lysates Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See S6505-08O-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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