S6504-36A

Src (Tyr416) (pp60Src, pp60c-Src)

Size

£691.00

SKU:: S6504-36A
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to residues surrounding Tyr416 of human Src.
Clone:: 3A6(7G9)
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are important in the regulation of growth and differentiation of eukaryotic cell. Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of tyrosine 416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of tyrosine 527 in the C-terminal tail by Csk renders the enzyme less active. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.