S6501-HRP

Src (pp60Src, pp60c-Src) (HRP)

Size

£1,182.00

SKU:: S6501-HRP
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chicken Src, purified from extracts of E. coli containing the plasmid plac-src.
Clone:: 0.T.138 (EC10)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Src is a member of the Src family of protein tyrosine kinases. This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets. Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins. Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor. In addition, Src is thought to play a role in physiological/ pathophysiological processes in the central nervous system. It has also been reported that mice deficient in Src develop severe osteoporosis. A total of eight membrane-associated protein tyrosine kinases have been identified within the Src gene family. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr whose roles include regulation of cytoskeletal organization, cell-cell contact, DNA synthesis, proliferation and differentiation of eukaryotic cells. Elevated activity of the proto-oncogene Src has been found in many human cancers, most notably breast cancer. The highest levels of expression of the prototype member of this gene family, Src p60, are observed in platelets and in neuronal tissues. With the fact that platelets have constitutively high levels of phosphotyrosine-containing proteins suggest that Src p60 may be important for platelet homeostasis. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml detected Src (60kD) in a RIPA lysate of chicken embryo fibroblasts (CEF). Immunoprecipitation: 1-2ug immnoprecipitated Src using 250-500ug of Src-transformed chicken cell extract, as reported by an independent laboratory. Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice