S6500-11A

Src, active, Recombinant, Human, GST-Tag (pp60Src, pp60c-Src)

Size

£912.00

SKU:: S6500-11A
Molecular Weight:: 86
Purity:: ~60%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Extra Details:: Recombinant full-length human SRC with N-terminal GST tag (Gene accession number NM_005417), expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: ~55 pmol/min/ug. Assay condition: 50mM HEPES (pH7.5), 10mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200uM ATP, 2uM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.