S6500-06C
Src (pp60Src, pp60c-Src)
Size
£626.00
- SKU:
- S6500-06C
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues near the C-terminus of human Src.
- Clone:
- 5i131(36D10)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) is important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity. Phosphorylation of Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: (Paraffin) 1:800 EDTA/TBST IHC Protocol Immunocytochemistry: (IF) 1:100 Immunoprecipitation: 1:50 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice