Sprouty 3 (SPRY3, Sprouty homolog 3 (Drosophila) HSPRY3, Sprouty homolog 3, Spry-3)

Size

£876.00

SKU:: S5620-04
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant human SPRY3 Met1-Glu142, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288aa in length and contains one SH2-binding domain (aa 25-30), a PEST sequence (aa82-93), and a Cys-rich domain (aa147-270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.