S5620-01G
Sprouty 1 (hSPRY1, Sprouty Homolog 1 Antagonist of FGF Signaling, Protein Sprouty Homolog 1, Sprouty Homolog 1, SPRY1, Spry-1)
Size
£642.00
- SKU:
- S5620-01G
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to CPSRGQGKPS, from SPRY1, at C-terminal (NP_005832.1, NP_955359.1).
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- SPRY1 is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-g 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319aa in length. It contains one CBL-TKB binding site aa51-57 that is phosphorylated at Tyr53, a Ser-rich region aa112-131, and a Cys-rich domain aa181-306 that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa1-178, human SPRY1 shares 76% aa identity with mouse SPRY1. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml Immunohistochemistry (Paraffin): 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice