S5525A
Spinophilin (Neurabin-II, PPP1R9B)
Size
£863.00
- SKU:
- S5525A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to aa289-412 from human PPP1R9B expressed in E. coli (Q96SB3).
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- PPP1R9B is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89kD, it runs anolamously at 120-140kD in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815aa in length. It contains one actin-binding region aa1-154, a PP1 binding domain aa415-492, a PZD domain aa493-583 and a coiled-coil region aa647-815. PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa291-414, human PPP1R9B shares 91aa identity with mouse PPP1R9B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice