S5525
Spinophilin (Neurabin-II)
Size
£960.00
- SKU:
- S5525
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- GST fusion protein corresponding to residues 286-390 of rat brain Spinophilin.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- Spinophilin/Neurabin-II has been found to be enriched at the postsynaptic density fraction in the brain and in the adherens junction fraction in rat liver. Within the brain, Spinophilin/Neurabin-II is localized in the heads of dendritic spines. Spinophilin/Neurabin-II contains a single PDZ domain in the middle region of the protein and other domains that predict it will form a coiled-coil structure at the C-terminus. Spinophilin/Neurabin-II is known to complex with the catalytic subunit of protein phosphatase I (PP1) and in vitro is a potent modulator of PP1 enzymatic activity. Spinophilin/Neurabin-II is also known to interact with F-actin and rat lin-10. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.25-1ug/ml detects Spinophilin in 20ug of a rat brain microsomal preparation. Immunoprecipitation: 4ug immunoprecipitates Spinophilin from 500ug of rat brain microsomal preparation. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice