S5440-50D

S-phase Kinase-associated Protein 2 (SKP2, Cyclin-A/CDK2-associated Protein p45, F-box Protein Skp2, F-box/LRR-repeat Protein 1, FBL1, FBXL1, FLB1, MGC1366, p45skp2)

Size

£754.00

SKU:: S5440-50D
Application:: IHC-Fr
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant corresponding to aa221-425 of human SKP2.
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.
Extra Details:: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.