S5440-50C

S-phase Kinase-associated Protein 2 (SKP2, Cyclin-A/CDK2-associated Protein p45, F-box Protein Skp2, F-box/LRR-repeat Protein 1, FBL1, FBXL1, FLB1, MGC1366, p45skp2)

Size

£512.00

SKU:: S5440-50C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a sequence from the N-terminal of human S-phase Kinase-associated Protein 2. Species Sequence Homology: rat and mouse (different by three amino acids)
Format:: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Extra Details:: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot:1ug/ml Immunohistochemistry:1-2ug/ml detects SKP2 in formalin fixed and paraffin embedded tissues. Immunocytochemistry:1-2ug/ml detects SKP2 in acetone fixed cells. Antigen retrieval by pepsin and trypsin is required. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice