Speedy-1 (SPDY1, SPY1, Speedy Homolog A, SPDYA, MGC110856, MGC57218, Rapid Inducer of G2/M Progression in Oocytes A, Ringo3)

Size

£594.00

SKU:: S5383
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide made to a C-terminal region of the human Speedy protein (within residues 200-286). This immunogen is conserved in both isoforms 1 and 2 [Swiss-Prot Q5MJ69].
Format:: Tris-citrate/phosphate (pH 7-8)
Extra Details:: Speedy/Ringo (Spy1) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. It mediates cell survival during the DNA damage process through activation of CDK2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Mouse testis lysate Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.