S5331-52B
Somatostatin Receptor Type 2 (SSTr2)
Size
£856.00
- SKU:
- S5331-52B
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Ile369 from human Somatostatin Receptor Type 2, expressed in NS0 cells.
- Clone:
- 8B44
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Somatostatin Receptor 2 (SSTr2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTr2 shares 84% aa identity with mouse SSTr2 within the extracellular domains. Isoform B (357aa) has an alternate C-terminal cytoplasmic region that is 12aa shorter than that of isoform A (369aa, reported as 93kD). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry (Intercellular staining): 2.5ug labels 10e6 cells. MDA-MB-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml in immersion fixed MDA-MB-231 human breast cancer cell line. Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human brain (cingulate cortex). Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice