S5329-36D
Solute Carrier Family 22 (Organic Cation Transporter) Member 1, SLC22A1)
Size
£787.00
- SKU:
- S5329-36D
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Thr554 from human Solute Carrier Family 22 expressed in NSO cells.
- Clone:
- 11D109
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80kD 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice