SLC12A6 (Solute Carrier Family 12 Member 6, Electroneutral Potassium-chloride Cotransporter 3, K-Cl Cotransporter 3, KCC3)

Size

£642.00

SKU:: S5329-13L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to SRHIDVCSKTKE, from human SLC12A6, at the internal region of the protein (NP_001035959.1, NP_001035960.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml, observed in human heart and kidney lysates at ~120kD bands Immunohistochemistry (Paraffin): 2-4ug/ml Paraffin embedded human heart shows staining of myocardial fibrils in transverse section. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.