solute carrier family 9 (sodium/hydrogen exchanger, APNH, APNH1, FLJ42224, Na(+)/H(+) antiporter, amiloride-sensitive, Na(+)/H(+) exchanger 1, NHE1, NHE-1, Sodium/hydrogen exchanger 1, Solute carrier family 9 member 1)

Size

£655.00

SKU:: S5329-10A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa from near the center of the human Nhe-1 (GenBank accession no. P19634).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane- bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters (1). Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger (2). It is also thought that Nhe-1 play a role in hypertension (3). At least two isoforms of Nhe-1 are known to exist. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1–2ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice