SODD (Silencer of Death Domains)

Size

£537.00

SKU:: S2000-06
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to amino acids 443-457 of human SODD protein
Format:: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Extra Details:: Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml Whole cell lysate from HeLa cells can be used as positive control. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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