S1040-01C
SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)
Size
£735.00
- SKU:
- S1040-01C
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein derived from the N-terminal region of mouse SOCS 1. Species Sequence Homology: human, rat; 95%.
- Clone:
- 4H314
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- SOCS (Suppressor of Cytokines Signaling) proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domain-containing protein. It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFNg), and erythropoietin (2). It plays an important role in the negative regulation of IFNg signaling and in the differentiation of T cells (3). SOCS 1 mRNA is highly expressed in the thymus, to a much lower level in the spleen, and not expressed in most other tissues (4). The SOCS 1 protein is primarily expressed in the thymocytes independent of the cytokine and T cell receptor (5). Biochemical characterization as well as gene disruption studies indicate that SOCS 1 is an important negative regulator of the JAK-STAT signal pathway. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: Immunogen only Immunohistochemistry (paraffin): 1:20. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice