S1014-59

SmiI (SwaI)

Size

£307.00

SKU:: S1014-59
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: 5'-A T T T^A A A T-3' 3'-T A A A^T T T A-5' Source: Streptococcus milleri S Concentration: 10units/ul Unit Definition: One unit is defined as the amount of SmiI required to digest 1ug of Ad2 DNA-SspI fragments in 1 hour at 30°C in 50ul of assay buffer. Storage Buffer: 10mM Tris-HCl (pH 7.4, 25°C) 200mM NaCl, 1mM DTT, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Supplied With: R1625 Restriction Enzyme Buffer A: 10X: 10mM Tris-HCl (pH 7.4, 25°C) 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used. R1625-03 Restriction Enzyme Buffer D: 10X: 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.