S1014-58V4-BIOTIN
Variola Virus B5R (Smallpox) (Biotin)
Size
£704.00
- SKU:
- S1014-58V4-BIOTIN
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa33-47, IQEYNLIRTYKKVDK of B5R protein. Species Sequence Homology: human, chimpanzee, canine, rat, mouse, chicken, zebrafish; 100%, Xenopus, 92%.
- Clone:
- 11E345 (1J2F3)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
- Extra Details:
- B5R is a 561-amino acid membrane protein containing ankyrin repeats that mediate protein-protein interactions and is related to the regulators of complement activation (RCA) superfamily. It consists of four short consensus repeats (SCRs) and transmembrane anchor region or the CT (cytoplasmic tail). Its transmembrane domain is the major determinant for targeting the B5R protein to the outer membrane of EEV (extracellular enveloped virus) and for supporting EEV formation, thus playing an important role in the process of virus envelopment and release. It may also be involved in viral evasion from host immune responses. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice