Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3) (Not for Export EU)

Size

£800.00

SKU:: S1014-53P2
Application:: WB
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic phosphopeptide corresponding to residues surrounding Ser423/425 of human Smad3. Cellular Localization: Cytoplasm. Species sequence homology: human and rat.
Format:: Supplied as a liquid in 0.05% sodium azide.
Extra Details:: Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 was used to detect Smad3 in UV-treated AKR-2B cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice