S1014-53J
Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Size
£787.00
- SKU:
- S1014-53J
- Purity:
- Purified by epitope affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
- Extra Details:
- Signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily are known to be mediated by SMAD proteins. Biological activities mediated by SMADs include cell growth and morphogenesis, development, and immune response. Activation by TGF-b is mediated by Smad2 and Smad3 while activation by bone morphogenetic protein (BMP) is mediated by Smad 1 and Smad 5. In contrast to the activating effects of these Smad proteins, Smad6 and Smad7, whose expression is also induced by ligand binding, appear to function as negative regulators of TGF-b superfamily signaling pathways. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunohistochemistry: 1ug/ml Requires HIER with EDTA, pH 8.0 prior to staining. Optimal dilutions to be determined by the researcher. Positive Controls: Western Blot: human Smad3-transfected COS Immunohistochemistry: human normal breast, esophagus, skin, and breast cancer tissues. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice