S1014-49
Smac, CT (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)
Size
£655.00
- SKU:
- S1014-49
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Raised against a peptide (SDEGADQEEEAYLRED) corresponding to amino acids 222 to 237 of murine Smac/DIABLO (2).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3–6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher. Positive Control: Mouse heart tissue lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice