S1014-29B
Sm (Smith) Antigen
Size
£960.00
- SKU:
- S1014-29B
- Purity:
- Purified by Protein G affinity chromatography from ascites
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- MW of Antigen: ~25kD (predominant). Cellular Localization: Nuclear
- Clone:
- 3F332
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S1014-29BX
- Extra Details:
- The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE. Applications: Suitable for use in Electron Microscopy, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml for 2 hours at RT Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minute at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher. Positive Control: LS174T cells, Tonsil, Testis Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice