SLAIN Motif-containing Protein 1 (SLAIN1, C13orf32)

Size

£837.00

SKU:: S1014-18D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Met1-Lys120 from human SLAIN1, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: SLAIN1 (SLAIN motif-containing protein 1) is a 61kD (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579aa in length. It contains a coiled-coil region (aa21-56), a Pro-rich segment (aa64-160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50aa insertion after Gln231. Over aa262- 483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. Validated with lysates of HeLa human cervical epithelial carcinoma cell line. Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human B-cell lymphoma. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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