SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3 Mitochondrial, Silent Mating Type Information Regulation 2 S. cerevisiae Homolog 3, Sir2-like 3, SIR2 Like Protein 3, SIR2-like Protein 3, SIR2L3)

Size

£642.00

SKU:: S1013-88F5
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C Terminus of human SIRT3(NP_036371.1, NP_001017524.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:128,000 Western Blot: 0.1-0.3ug/ml ~28kD band observed in Human Liver lysates (calculated MW of 28.6kD according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kD and 48kD are observed. These bands are not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice