S1013-87Q-BIOTIN

SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (Biotin)

Size

£960.00

SKU:: S1013-87Q-BIOTIN
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat peritoneal macrophages
Clone:: OX-41
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Optimal dilution determined by the researcher Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed) Immunohistochemistry: Recommended Secondary Reagents: Goat anti mouse IgG:HRP (rat adsorbed) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.