S1013-72K
SNF1LK2 (SNF1-like kinase 2, DKFZp434K1115, KIAA0781, LOH11CR1I, QIK, Qin-induced kinase, Serine/threonine-protein kinase SNF1-like kinase 2, SIK2)
Size
£837.00
- SKU:
- S1013-72K
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Ala663-Leu818 from human SNF1LK2.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- SNF1LK2 (Sucrose non-fermenting 1 like kinase 2) also known as SIK2 (Salt-inducible kinase 2) is a cytoplasmic 125 kDa member of the CAMK Ser/Thr protein kinase family, AMPK subfamily of enzymes. It is expressed in preadipocytes/adipocytes, with levels strongly induced during early differentiation. SNF1LK2 regulates the early phase of insulin signaling by phosphorylating IRS-1 on Ser794. Human SNF1LK2 is 926 amino acids (aa) in length and contains a protein kinase domain (aa 20‑271) with an ATP binding site (Lys49) and a catalytic loop (aa 140‑149), followed by a phosphorylation activation site at Thr175, a UBA (ubiquitin-associated) domain (aa 295‑335) and another PKA-dependent phosphorylation site at Ser587. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice