S1013-57E

Siglec 1 (Sialic Acid Binding Ig-like Lectin 1, CD169, CD169 Antigen, Sialic Acid-binding Ig-like Lectin 1, Sialic Acid-binding Ig-like Lectin-1, Sialoadhesin, Sialoadhesin Precursor, Siglec 1, SN)

Size

£659.00

SKU:: S1013-57E
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Porcine alveolar macrophages
Clone:: 10B853 (3B11/11)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Siglec-1 cell surface antigen, also known as CD169, is expressed by subsets of cells of the macrophage/ monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Porcine spleen Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.